Fig. 3
RT heterogeneity fluctuates throughout S phase and peaks in the middle of S phase. a Schematic showing the fitting of a sigmoidal curve on the bin wise cumulative percentage replicated plotted against time into S phase. Example datapoints (blue) of cumulative percentage of replication in high-resolution Repli-Seq heatmap. Black line represents the fitted sigmoidal curve. Green dotted line represents trep , which is the S phase time at which replication is 50% completed in the cell population. Distance between grey dotted lines represents twidth, which is the time it takes for replication to be from 25% replicated to 75% replicated in the cell population. b (i) Cumulative replication percentage for IZs of early (red), early-mid (blue), late-mid (green) and late (black) timing where each line represents an individual IZ. b (ii) Scatter plots showing the relationship between Twidth and Trep calculated from fitted sigmoidal curves for early (red), early-mid (blue), late-mid (green) and late (black) IZs in H1, H9, HCT116 and F121-9 mESC. b (iii–vi) Scatter plots showing the relationship between Twidth and Trep for TTRs (iii), breakages (iv), termination sites (< 100 kb) (v) and late CTRs (vi) in H1, H9, HCT116 and F121-9 mESC