Fig. 3

The impact of dropouts on isoform detection. a The distribution of the probabilities of dropouts (p(dropout)) in each group of H1 hESCs and an approximation of these distributions using a beta distribution. At 1 million reads per cell, α = 1.31 and β = 0.74 in the approximated beta distribution. At 4 million reads per cell, α = 0.72 and β = 1.03 in the approximated beta distribution. b Five beta distributions from which dropout probabilities were sampled from the simulations used to generate c and d. In c, the distribution of the mean number of isoforms detected per gene per cell is shown for simulations in which one isoform was produced per gene per cell. Each plot corresponds to a simulation in which dropout probabilities were sampled from one of the distributions shown in b. d The overlap fraction for each simulation. Plots shown in c and d are for H1 hESCs sequenced at 4 million reads per cell. Black vertical lines represent the mean value of the distributions