Fig. 8

Hyperthermophiles have weak ∆LFE. a ∆LFE profiles (for CDS beginning and end) for members of euryarchaeota covered by the phylogenetic tree (N = 28) and their annotated optimum growth temperature classification (mesophile—green, moderate thermophile—orange, hyperthermophile—red) and genomic GC-contents. Hyperthermophiles have weak ∆LFE that cannot be explained by the tree topology or their genomic GC-contents. b ∆LFE profiles (left) and optimum growth temperatures (right) for all members of euryarchaeota having annotated optimum growth temperatures (N = 25), plotted using their PCA coordinates (see “Visualization” under the “Methods” section). Hyperthermophiles seem to be clustered in a small region characterized by weak ∆LFE. c ∆LFE profiles (left) and optimum growth temperature (right) for all species having annotated optimum growth temperature (N = 173), plotted using their PCA coordinates (see “Visualization” under the “Methods” section). Short species names from PCA plots are listed in Additional file 1: Table S3. d Comparison of ∆LFE values for species having optimum temperature above (blue) or below 75 °C (yellow), for positions relative to CDS start (left) or end (right). e Regression for optimum growth temperature vs. mean ∆LFE (average for positions 100–300 nt after CDS start) using GLS (green regression line, N = 96, R² = 0.004, p value = 0.6) and OLS (red regression line, N = 173, R² = 0.45). The apparent linear relation is no longer significant when controlling for the phylogenetic relationships. Points plotted in red are included only in OLS