Fig. 1

H3K27ac is dramatically decreased at enhancers in H3.3K27R mutant cells. a Western blot of H3K27ac from WT and H3.3K27R mutant mouse ESC lines. b Volcano plot illustrating the H3K27ac signal changes in H3.3K27R mutant ESCs in comparison with WT ESCs. Inlet diagram representing peak numbers in different categories of fold changes. FDR, false discovery rate. c Bar plot of the percentages of significantly decreased H3K27ac peaks at enhancers and promoters, respectively. d, e H3K27ac signals in WT and H3.3K27R mutant mouse ESC lines at enhancers (d) and TSSs (e). Upper, averaged profiles of the H3K27ac ChIP-seq signal at enhancers and TSSs, respectively. Lower, heatmap plots of the H3K27ac ChIP-seq signal at enhancers and TSSs, respectively. All biological duplicates are shown. f Genome browser representations of H3K27ac ChIP-seq, mRNA-seq, and ATAC-seq in WT and H3.3K27R mutant ESC lines at the Pou5f1 and Nanog loci. Super-enhancer regions of each gene are highlighted with dashed line boxes