Fig. 2From: Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptidesG8PPD prevents Cas9-sgRNA assembly. a Dose-dependent inhibition of Cas9-sgRNA binding by f1 G8PPD. sgRNA concentration is fixed to 15 μM. b f1 G8PPD prevents Cas9-sgRNA assembly prior to, but not post, sgRNA addition. Cas9 to sgRNA ratio is fixed to 0.3. The above results are shown as mean ± SD (n = 3)Back to article page