Fig. 1

scRNA-seq quality metrics remain stable for at least 24 h of cold storage. a Experimental design: samples from the lung, esophagus, and spleen were collected from 5 or 6 donors each and stored as whole organ pieces at 4 °C for different time points prior to tissue processing for scRNA-seq and bulk RNA-seq. b–e Change of quality metrics of scRNA-seq data obtained with time, showing the b number of reads per sample, c number of cells per sample, d median number of genes detected per cell, and e number of genes confidently mapped to the transcriptome