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Table 4 Comparison of different assemblies of barley cv. Morex

From: TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools

 

BAC-by-BAC

TRITEX

TRITEX

Morex V1 [3]

Dovetail

Morex V2

MP9 only

Scaffold assembly size

4.79 Gb

4.65 Gb

4.6 Gb

Scaffold N50

79 kb

3.4 Mb

2.6 Mb

Scaffold N90

4.4 kb

287 kb

150 kb

Assembled sequence in scaffolds ≥ 1 kb

4.67 Gb

4.34 Gb

4.32 Gb

Assembled sequence in scaffolds ≥ 1 Mb

0 bp

3.80 Gb

3.49 Gb

Unfilled internal gaps

216 Mb (4.5%)

116 Mb (2.5%)

106 Mb (2.3%)

Super-scaffold N50

1.9 Mb

1.3 Mb

40.2 Mb

32.6 Mb

Super-scaffold N90

336 kb

7.5 kb

2.0 Mb

1.2 Mb

Size of pseudomolecules

4.58 Gb

 

4.26 Gb

4.20 Gb

Size of unanchored sequences (chrUn)

246 Mb

 

83 Mb2

111 Mb2

Proportion of complete full-length cDNAs1

81.8%

84.1%

89.8%

90.4%

  1. 1Proportion of 28,622 full-length cDNAs of barley cv. Haruna Nijo [42] aligned with ≥ 90% coverage and ≥ 97% alignment identity
  2. 2Sequences shorter than 1000 kb were not included in chrUn