Fig. 2

Example of a chimeric scaffold. The chimeric nature of a sequence scaffold joining two unlinked sequences originating from barley chromosomes 2H and 5H is supported by multiple lines of evidence. a Genetic chromosome assignments of marker sequences aligned to scaffold_1005. b 10X molecule coverage. c Physical Hi-C coverage. Coverage in b and c was normalized for distance from the scaffold ends and the log2-fold observed vs. expected ratio was plotted. The red, dotted lines mark the breakpoint at 3.32 Mb