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Table 1 Summary of individuals included in this study

From: NanoSatellite: accurate characterization of expanded tandem repeat length and sequence through whole genome long-read sequencing on PromethION

IndividualABCA7 VNTR lengths (kb)DNA sourcePromethION deviceSequencing chemistryNumber of flow cellsYield (Gb)Read length N50 (kb)
Subject015.14.5QIAmpα1086 + 7d74.47.0
Subject021.81.8QIAmpα108161.311.2
Subject034.72.3QIAmpα109172.516.3
Subject049.70.8QIAmpα109185.511.5
Subject0510.71.7QIAmpα109188.511.9
Subject062.30.3QIAmpα109198.016.2
Subject073.33.3Magtrationα109177.710.6
Subject082.30.4Magtrationβ109156.214.7
Subject09a5.54.3Magtrationβ109143.229.3
Subject10b8.72.8Magtrationβ109148.99.1
NA192404.52.2Magtrationβ109c5220.015.8
  1. DNA was either extracted with QIAmp (Qiagen) or on a Magtration robotic platform (PSS). An alpha (α) and beta (β) PromethION device were used. Two chemistries were applied: SQK-LSK108 (108) or SQK-LSK109 (109). Yield and mean read length were calculated with NanoPack [23] after Albacore or Guppy base calling. kb kilobases, Gb gigabases, N50 50% of the total sequencing dataset is contained in reads equal or larger than this value. aDNA was not sheared for this individual. bAn 8-year-old DNA extraction was used. cSQK-LSK109 was used for four flow cells and for one flow cell was run with SQK-LSK108. dSix debug PromethION flow cells and seven MinION flow cells were used