Fig. 3

Nucleosome counts depend on the level of digestion and are not proportional to nucleosome occupancy. IGV browser snapshot (chromosome 2L: 4,817,130–4,819,270) showing the normalized mononucleosomal read counts that are obtained after digesting the nuclei with MNase for various intervals of time (indicated on the left side of each track). The seventh track, shown as a heat map, represents the variation of A/T content along the genome. White color represents the genome-wide average A/T content (~ 0.58), red color represents A/T-rich regions, and blue color represents G/C-rich regions. The identified peaks and their classification are shown in the last track. Note that peaks that disappear very rapidly (e.g., 1) may correspond to non-histone complexes, such as nucleosome remodelers [29]