Fig. 2

Disrupting histone modifications affects expression of key circadian clock regulators. a ChIP-seq assays showing decreased binding of H3K4me3 to the genic regions of CCA1 (top) and LHY (bottom) in the sdg2 mutant compared with the wild type (WT). Green arrows next to the gene diagrams indicate transcriptional direction. b Relative expression levels (R.E.L.) of CCA1 (top) and LHY (bottom) in the sdg2 mutant compared with the WT at ZT0, ZT12, and ZT24 under a diurnal cycle (16 h light/8 h dark). Y-axis was broken as a result from large variation of expression levels. c ChIP-seq assays showing increased binding of H3K4me3 to the genic regions of CCA1 (top) and LHY (bottom) in the jmj14 mutant compared with the WT. d R.E.L of CCA1 (upper) and LHY (lower) in the jmj14 mutant compared with the WT under a diurnal cycle (16 h light/8 h dark). Error bars in b and d indicate standard deviation of biological replicates (n = 3) with an asterisk showing the statistical significance level (P < 0.05, Student t test)