Fig. 5
From: Alternative splicing is required for stage differentiation in malaria parasites
Exflagellation is inhibited in PbSR-MG KO parasites and restored in complemented parasites. a Immunofluorescence assay of male gametocytes induced into exflagellation. Green represents a male gametocyte-specific GFP used for sorting. Red and blue indicate alpha-tubulin and DNA, respectively. Wild-type parasites often exhibited conventional exflagellation, as shown in the top row. Very few PbSR-MG KO parasites were able to exflagellate (Fig. 4d), as represented in the bottom row. b Complementation of PbSR-MG KO parasites with full-length PbSR-MG on artificial chromosome PbAC0281c08. Exflagellation assay comparing WT, KO ΔDH, and KO ΔDH+PbAC (p value < *0.05; **0.01, ***0.001, ****0.0001)