Fig. 1
Transcriptomic and epigenomic profiling of teloHAEC. a RNAseq of teloHAEC non-treated (NT) or treated with TNFα identified 1316 differentially expressed (DE) genes (FDR < 0.1% and absolute log10-fold-change > 0.3) among three comparisons (NT vs. 4 h, NT vs. 24 h, 4 h vs. 24 h). Of these 1316 genes, 836 genes were DE in the NT vs. 4 h comparison. b Gene expression fold-change for DE genes are highly correlated between transformed teloHAEC and primary HCAEC. All three comparisons are highly significant (P < 2.2 × 10−16), but for simplicity we only show the NT vs. 4 h comparison. c ATACseq of teloHAEC NT or treated with TNFα identified 95,491 peaks, including 3138 differentially opened (or closed) (DO) peaks (FDR < 0.1% and absolute log10-fold-change > 0.3) among three comparisons (NT vs. 4 h, NT vs. 24 h, 4 h vs. 24 h). Of these 3138 peaks, 2654 peaks were DO in the NT vs. 4 h comparison. d Open chromatin regions (raw number of reads), identified by ATACseq, are highly correlated between teloHAEC and HCAEC. Results shown are for the 4 h TNFα treatment. Results are consistent for the NT and 24 h timepoints. The distribution falls under the diagonal because the coverage of the ATACseq teloHAEC libraries was higher than the coverage of the HCAEC libraries