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Fig. 5 | Genome Biology

Fig. 5

From: Translation of the circular RNA circβ-catenin promotes liver cancer cell growth through activation of the Wnt pathway

Fig. 5

Circβ-catenin has the protein-coding capacity. a Schematic illustration of the circularization of circβ-catenin. b The predicted sequence of β-catenin-370aa. c After pulled down by using a β-catenin antibody, the protein samples from PLC/PRF/5 cells were subject to mass spectrometry analysis. And the specific peptides from β-catenin-370aa were identified. d The expression levels of full-length β-catenin and β-catenin-370aa were examined by western blotting after stable knockdown of circβ-catenin in PLC/PRF/5 cells. e Validation of β-catenin-370aa with HA tag by western blotting. f Immunostaining was conducted to examine the cellular location of β-catenin-370aa in HEK293 cells. g The putative IRES activity in circβ-catenin was tested by Dual-Luciferase Reporter Assay (*P < 0.05, **P < 0.01)

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Genome Biology

ISSN: 1474-760X

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