Table 1 Virus infection results
From: Linking CRISPR-Cas9 interference in cassava to the evolution of editing-resistant geminiviruses
Time post inoculation | Genotype | Line number | Proportion of symptomatic plants | % of symptomatic plants | Mean symptom severity of symptomatic plants (0–3) | % mutant (AC2-H54Q) virus of total |
|---|---|---|---|---|---|---|
3 weeks | Wild-type | – | 5/9 | 55.5 | 2 | 0 |
Cas9 | 154 | 0/5 | 60 | 1.4 | 0 | |
155 | 4/6 | 66.6 | 1.7 | 0 | ||
Cas9+sgRNA | 92 | 1/8 | 12.5 | 2 | 0 | |
111 | 5/5 | 100 | 2.8 | 0 | ||
114 | 6/6 | 100 | 2.3 | 0 | ||
118 | 3/5 | 60 | 1.3 | 0 | ||
120 | 1/9 | 11.1 | 1 | 0 | ||
130 | 1/7 | 14.3 | 3 | 0 | ||
139 | 0/5 | 0 | 0 | 0 | ||
8 weeks | Wild-type | – | 6/9 | 67 | 1.8 | 0 |
Cas9 | 154 | 3/5 | 60 | 2.3 | 0 | |
155 | 6/6 | 100 | 2.2 | 0 | ||
Cas9+sgRNA | 92 | 8/8 | 100 | 1.9 | 0 | |
111 | 5/5 | 100 | 2.6 | 0 | ||
114 | 6/6 | 100 | 1.8 | 0 | ||
118 | 4/5 | 80 | 1.7 | 5.2 | ||
120 | 7/9 | 78 | 1.7 | 0 | ||
130 | 6/7 | 86 | 2.5 | 2.2 | ||
139 | 1/5 | 20 | 3 | 1.6 |