Fig. 1
From: Linking CRISPR-Cas9 interference in cassava to the evolution of editing-resistant geminiviruses
sgRNA design and expression profiling of CRISPR/Cas9 transgenics. a Low off-target sgRNAs targeting the DNA A of the African cassava mosaic virus and in silico predicted efficiency score (max. efficiency= 1). b In vitro cleavage assay for testing the effectiveness of six different sgRNAs against the viral template. c, d Reverse transcription-quantitative PCR (RT-qPCR) analysis of Cas9 and sgRNA transgene expression respectively. Three independent plants per line were tested