Fig. 6

The streamlined BRB-seq data analysis workflow and its low cost. a Schematic representation of the BRB-seq library post-sequencing data processing pipeline. It includes the BRB-seqTools module (available on github, see the “Methods” section) that can perform optional read trimming, alignment, sample demultiplexing, and generation of a count table. The count table can be further analyzed by standard algorithms or loaded into ASAP, a web-based analytical interface that facilitates data exploration and visualization. b The estimated per sample cost of library preparation for 96 samples for TruSeq and BRB-seq. Per sample cost of BRB-seq involving in-house made Tn5 or Nextera Tn5 is indicated