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Fig. 2 | Genome Biology

Fig. 2

From: BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing

Fig. 2

Schematic overview of the BRB-seq protocol. This schema highlights in details all steps of the final BRB-seq protocol. The bottom grayed window shows the final BRB-seq construct used for Illumina sequencing. The read Read1 and Read2 primers are used to sequence the barcode/UMI and cDNA fragment respectively. Index read (i7) is used to demultiplex Illumina libraries. Legend: DS cDNA, double-stranded cDNA

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Genome Biology

ISSN: 1474-760X

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