Fig. 6

A3G suppresses mitochondrial basal respiration relative to glycolysis without inhibiting proliferation in crowded HuT78 cells. a Respiration/glycolysis (R/G) ratios, first normalized to T0 unstressed controls, then to CTRL, which is set to 1, in five different Seahorse experiments show increased R/G ratio by A3G KD (p = 0.004 by t test, total n = 15 in five experiments). Exp1–3 are performed with KD1a; Exp4 and5 are performed with KD2. Seahorse assay is performed on unstressed (no editing) and stressed (after cellular crowding with 5–10 million/ml) in normoxia, which induces RNA editing. (n = 2–4 replicates per experiment; mean and SEM shown). b Cell proliferation changes upon induction of RNA editing by cellular crowding in CTRL, and KD2 HuT78 cells measured by thymidine incorporation assays. CTRL and KD T0 values are normalized to 10,000 CPM, and relative changes caused by crowding are shown. (p(exp1) = 0.008, p(exp2) = 0.41 by unpaired t test. Fisher’s combined probability test p value < 0.05, χ² = 11.44, df = 4)