Fig. 1
From: Identification of transcription factor binding sites using ATAC-seq
Cleavage enzymes of ATAC-seq and DNase-seq. Sequence motif relative to aligned read starts after cleavage with Tn5 and DNase I enzymes on naked DNA ATAC-seq (a) and DNase-seq (b) experiments. Position 1 corresponds to the start position of the ATAC/DNase-seq read. The size of the motifs is reflected by the structural protein contacts of Tn5 and DNase-I (Protein Data Bank entries 1MM8 and 2DNJ). c Tn5 inserts adapters in both DNA ends. Moreover, DNA is cleaved into two 9 bps single ends, which are later repaired in the ATAC-seq protocol