Fig. 1

CRISPR-Cas9 screens and cell ploidy effect. a Enrichment of non-specific CRISPR-Cas9 LOF effects in non-expressed genes (RNA-seq RPKM < 1) grouped by their copy-number profile, performed across 250 cell lines. For each copy-number group, the recall curve is drawn and the area under the recall curve (AURC) is reported. X-axis shows the ranked gene level CRISPR-Cas9 fold changes, from negative to positive. b Boxplots of AURCs as in a but performed in each cell line independently. Each dot represents the AURC of the given gene copy-number in a specific cell line. c Similar to b but cell lines are grouped according to their ploidy status. d AURC of non-expressed genes estimated per chromosome in each cell line independently. Chromosomes were grouped according to their estimated number of copies. Boxplots represent 1.5 of the interquartile range