Fig. 2
From: Gene editing of the multi-copy H2A.B gene and its importance for fertility
H2A.B.3 KO males have impaired fertility. a The mRNA level of each H2A.B.3 gene was determined by real-time quantitative PCR, relative to HPRT. Primers were designed to anneal within the TALEN-induced deletions. b Confirmation that H2A.B.3 protein is not produced in H2A.B.3−/y mice. H2A.B was immunoprecipitated from total testis lysates followed by Western blotting using anti-H2A.B.3 polyclonal antibodies. c The breeding was conducted using age-matched wt females (3–6 months) and age-matched wt (n = 5) and H2A.B.3−/y (n = 5) males. Total number of pups produced was 201 and 256 for wt and KO males, ***p value = ≤ 0.001 (ANOVA test)