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Fig. 2 | Genome Biology

Fig. 2

From: Guide RNAs with embedded barcodes boost CRISPR-pooled screens

Fig. 2

Schematic of CRISPR-pooled screening using sgRNAiBAR. For a given sgRNAiBAR library, four different iBAR6s were randomly assigned to each sgRNA. The sgRNAiBAR library was introduced into target cells through lentiviral infection with a high MOI (i.e., ~‚ÄČ3). After library screening, sgRNAs with their associated iBARs from enriched cells were determined through NGS. For data analysis, median ratio normalization was applied, followed by mean-variance modeling. The variance of sgRNAiBAR was determined based on the fold change consistency of all iBARs assigned to the same sgRNA. The P value of each sgRNAiBAR was calculated using the mean and modified variance. Robust rank aggregation (RRA) scores of all genes were considered to identify hit genes. A lower RRA score corresponded to a stronger enrichment of the hit genes

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