Fig. 5

miR-196a regulates HNC cell proliferation and survival by promoting G1/S transition and apoptosis resistance. a Cell proliferation assays with CAL 27 cells at 48 h after transfection with miR-196a or anti-miR-196a. b Representative micrographs and quantification of EdU-incorporating cells at 48 h after transfection with miR-196a or anti-miR-196a. c MTT assay of CAL 27 cells transfected with miR-196a or anti-miR-196a for 48 h, followed by cisplatin treatment at the indicated concentration for 72 h. d Plate colony formation assay of CAL 27 cells transfected with miR-196a or anti-miR-196a with cisplatin treatment (3 μM). e The cell cycle distribution was analyzed by a flow cytometer in CAL 27 cells transfected with miR-196a or anti-miR-196a at 48 h after transfection. f, g Flow cytometric analysis of cisplatin-induced (10 μM) apoptosis in CAL 27 cells transfected with miR-196a or anti-miR-196a at 48 h after transfection. (*p < 0.05; **p < 0.01; ***p < 0.001)