Fig. 7
From: NmeCas9 is an intrinsically high-fidelity genome-editing platform
Guide truncation can suppress off-target editing by NmeCas9. a Editing efficiencies at the NTS1C (on-target, red) and NTS1C-OT1 (off-target, orange) genomic sites, after editing by NmeCas9 and NTS1C sgRNAs of varying lengths, as measured by PCR and high-throughput sequencing. Data are mean values ± s.e.m. from three biological replicates performed on different days. b As in (a), but using sgRNAs perfectly complementary to the NTS1C-OT1 genomic site. Two-tailed paired Student’s T test showed significant difference in on- and off-target editing efficiency as a function of guide truncation. On-target editing (black asterisk) or off-target editing (red asterisk) is compared to the baseline condition GN24, respectively (p < 0.05 is annotated with one asterisk; p < 0.01 is annotated with two asterisks)