Fig. 6

miRTrace can be refined to trace samples to the species level. a miRNAs known to be specific to individual Drosophila species were compiled to generate a custom database for miRTrace. miRNA sequencing data from the 12 species were obtained from public repositories and assigned to the species by miRTrace, using the custom annotations. The number in each bar indicates the percentage of sequences that were traced to the correct species (pale yellow). b Sanger sequences of the COI gene were simulated for the 12 Drosophila species, considering intra-species variation and sequencing error rates, and assigned using the Barcode of Life Identification Engine. For each species, 1000 sequences were simulated. c RNA sequencing data from the 12 Drosophila species were obtained from public repositories and assigned using the FastQ Screen tool. The black asterisks indicate species where it was possible to obtain matched miRNA and RNA sequencing data (obtained from the same study)