Fig. 2

Correlation of annotations to functional scores. Data from Munoz et al. [7] is shown for representative cell line RKO. a Violin plot showing the distribution difference for guide RNA RKO z-scores targeting inside versus outside of predicted domains (as defined by InterPro). b Density plot showing the relation between RKO z-score and PROVEAN score (more negative is more conserved). c Density plot showing the relation between RKO z-score and disorder scores (1 equals disorder, 0 equals order). d Scatter plot showing the relation of median RKO z-score (x-axis), standard deviation (distribution) of PROVEAN score (marker size), and the median of the PROVEAN score (marker color) with the amount of correlation between PROVEAN scores and RKO z-scores (y-axis), for every gene. e Analogous to d, but for disorder score in place of PROVEAN score. f Heatmap showing the mean RKO z-score and the percentage guide RNAs falling into groups categorized based on domain annotation and conservation. g Heatmap showing the mean RKO z-score and the percentage guide RNAs falling into groups categorized based on conservation and disorder score. h RKO z-score distribution per amino acid. i RKO z-score distribution per non-mutually exclusive amino acid class: polar (S, T, Y, N, Q); nonpolar (G, A, V, C, P, L, I, M, W, F); hydrophobic (A, V, I, L, M, F, Y, W); hydrophilic (S, T, H, N, Q, E, D, K, R); positively charged (R, H, K); negatively charged (D, E); aliphatic (A, G, I, L, P, V); aromatic (F, W, Y); acidic (D, E); basic (R, H, K); hydroxilic (S, T); sulfur containing (C, M); and amidic (N, Q). j RKO z-score distribution per codon encoding for isoleucine (I). k Distribution of RKO z-scores for guides targeting amino acids with different predicted secondary structure: coil/unstructured, sheet, or helix. l Distribution for RKO z-scores for guides targeting sequences that are predicted to undergo or escape nonsense-mediated decay (NMD)