Fig. 6

Higher expression and increased stability for genes that shift to greater reliance on distal polyadenylation sites in quiescence. a For two different fibroblast lines (12–1 and 12–3), the log₂(7dCI counts/Proliferating counts) is plotted for genes that shift to increased use of more distal polyadenylation sites with quiescence and a control group that does not shift to more distal site use. Boxes indicate 25 to 75% ranges and whiskers indicate minimum and maximum values. Statistical significance was determined with Wilcoxon signed-rank test. The ratio of expression level in 7dCI versus P was higher for genes that shift to more distal polyadenylation site use with quiescence for both 12–1 and 12–3 fibroblasts. b Isoform-specific transcript decay half-lives were determined for 12–1 and 12–3 strains of fibroblasts in proliferating and quiescent conditions. Box plots show the range of half-lives for isoforms that terminate at proximal polyadenylation sites and isoforms that terminate at more distal polyadenylation sites in proliferating and quiescent conditions. Long isoforms are significantly more stable in quiescent but not proliferating states in 12–1 and 12–3 fibroblasts. Statistically significant differences were determined by Wilcoxon signed-rank test. c Density plots of half-lives for isoforms that terminate at proximal or distal polyadenylation sites in proliferating and quiescent fibroblasts from strains 12–1 and 12–3