Fig. 9

Effects of reducing CLANKs on Nv-twi expression. A1–B4 Wildtype expression of Nv-twi from early blastoderm through gastrulation. A1–A4 Wildtype embryos stained with DAPI to approximate embryo age. B1–B4 In situ hybridization of wildtype embryos probing for Nv-twi expression. Embryos B1–B4 correspond to same embryos in A1–A4. C1–F4 Altered expression of Nv-twi following pRNAi of one CLANK transcript (lower left corner) in early blastoderm through gastrulating embryos. C1–C4 and E1–E4 Knockdown embryos stained with DAPI to approximate embryo age. D1–D4 and F1–F4 In situ hybridization of knockdown embryos probing for Nv-twi expression (“phenotype” observed, lower right). Embryos correspond to the same embryos in C1–C4 and E1–E4. Most embryos are oriented with anterior to the left, posterior to the right, dorsal up, and ventral down (C4/D4, E2/F2-E4/F4 are bird’s eye ventral views)