Fig. 7

Effects of reducing CLANKs on Nv-zen expression. A1–B4 Expression of Nv-zen from early blastoderm through gastrulation in control embryos. A1–A4 Control embryos stained with DAPI to approximate embryo age. B1–B4 In situ hybridization of control embryos probing for Nv-zen expression. Embryos B1–B4 correspond to same embryos in A1–A4. C1–F4 Altered expression of Nv-zen following pRNAi of one CLANK transcript (lower left corner) in mid-late blastoderm embryos. C1–C4 and E1–E4 Knockdown embryos stained with DAPI to approximate embryo age. D1–D4 and F1–F4 In situ hybridization of knockdown embryos probing for Nv-zen expression (“phenotype” observed, lower right). Embryos correspond to same embryos in C1–C4 and E1–E4. All embryos are oriented with anterior to the left, posterior to the right, dorsal up, and ventral down