Fig. 8

HOXC-AS3 regulates expression of HDAC5. a Based on qRT-PCR assays, the level of HDAC5 was upregulated in 60 pairs GC tissues. The ΔCt value was determined by subtracting the GAPDH Ct value from the HDAC5 Ct value. A smaller ΔCt value indicates higher expression. b The level of HOXC-AS3 in GC tissues showed a significant positive correlation with the relative level of HDAC5 expression (n = 60). c Western blot assays detected the expression of HDAC5 after knockdown of HOXC-AS3 in BGC-823 cells. The promotion of HDAC5 (mRNA and protein) by HOXC-AS3 was significantly reversed by knockdown of YBX1, based on qPCR and western blot assays. d MTT assays showed that knockdown of HDAC5 could suppress cell proliferation, and knockdown of HDAC5 could also reverse HOXC-AS3-mediated growth promotion. e Proposed model in which HOXC-AS3 mediates the proliferation and migration progression of GC