Fig. 4

HOXC-AS3 regulates GC cell proliferation and migration in vivo. a Scramble or shHOXC-AS3 was stably transfected into BGC-823 and SGC-7901 cells, which were injected into nude mice, respectively. Tumor volumes were calculated after injection every 2 days. Tumor weights are represented as the means of tumor weights ± S.D. (standard deviation). The qRT-PCR was performed to detect the average expression of HOXC-AS3. Immunohistochemistry analysis revealed that the tumors developed from shHOXC-AS3 cells displayed lower Ki-67 staining than the control group. b Analysis of an experimental metastatic animal model was performed by injecting BGC-823 cells stably transfected with HOXC-AS3 knockdown into nude mice. Lungs from the mice in each experimental group, together with the number of tumor nodules on lung surfaces are shown. Visualization of the entire lung and HE-stained lung sections. *P < 0.05, **P < 0.01