Fig. 4
From: All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo
GUIDE-seq genome-wide specificities of NmeCas9. a Number of GUIDE-seq reads for the on-target (OnT) and off-target (OT) sites. b Targeted deep sequencing to measure the lesion rates at each of the OT sites in Hepa1–6 cells. The mismatches of each OT site with the OnT protospacers is highlighted (blue). Data are presented as mean ± SD from three biological replicates. c Targeted deep sequencing to measure the lesion rates at each of the OT sites using genomic DNA obtained from mice injected with all-in-one AAV8-sgRNA-hNmeCas9 sgPcsk9 and sgRosa26 and sacrificed at day 14 (D14) or day 50 (D50) post injection. Data are presented as mean ± SD