Fig. 2
From: Single-cell RNAseq for the study of isoforms—how is that possible?
Summary of limitations of the four ideal conditions for successful studies of single-cell RNAseq isoforms. From left to right, the importance and current limitations of full-length transcript sequencing, capture efficiency and sequencing depth, the number of cells sequenced, and sequencing errors and artefacts for isoform detection are presented in the diagram. Each is discussed in the main text. Alt. alternative, RT reverse transcription, UMI unique molecular identifier