Fig. 1
From: Cell type-specific CLIP reveals that NOVA regulates cytoskeleton interactions in motoneurons
BAC-transgenic mouse lines express AcGFP-tagged NOVA2 in MNs. a The generation of recombinant BAC. A-box, a ~ 500-nt sequence homologous to the mouse Chat 5′ UTR region, mediated the insertion of a plasmid containing GFP-Nova2 coding sequence downstream of Chat promoter. b Western blot of spinal cord lysate from wild type (WT) mice and mice from Chat:GFP-Nova2 #6 and #17 lines using human anti-NOVA serum. The 93-kD GFP-NOVA2 was expressed only in transgenic spinal cords. GAPDH was blotted as a loading control. c Immunofluorescence on spinal cord transverse sections using antibodies against CHAT and GFP, and counterstained with TO-PRO-3. Scale bar represents 50 μm