Fig. 3

Nascent RNA and miRNA expression changes upon aging in pro- and pre-B cells. a Nuclear RNA-seq from pro-B cells identifies significant changes in expression of nascent and non-coding transcripts between B cell precursor cells from young and aged mice. b Small RNA-seq from pro-B (left) and pre-B cells (right) identifies significant changes in expression of miRNAs between B cell precursor cells from young and aged mice. c Hierarchical clustering of miRNAs that are differentially expressed upon aging in pro- and/or pre-B cells identifies groups of co-regulated miRNAs. Several of these clusters also display developmental co-regulation from the pro-B to the pre-B cell stage. Horizontal black lines indicate the major clusters identified, with trends outlined on the left hand side of the heatmap. d A novel non-coding RNA encompassing Let-7b/c2 is differentially expressed between young and aged pro-B cells. Read counts were generated over 100-bp windows and normalized using size factors from DESeq2. Reads originating from the forward strand are represented as positive values (red bars), while those originating from the reverse strand are represented as negative numbers (blue bars). Genes are indicated at the top; shading indicates the location of the Let-7b and -c2 genes