Fig. 5

Histone 4 acetylation regions related to behavioral inter-individual variability. a Workflow of the analytical steps using histone H4 acetylation ChIP-seq. b Classification of the acH4 peaks obtained depending on their position relative to the transcription start site (TSS) of the nearest gene. c Histogram representing the relative positions of the acH4 peaks located around the TSS of the nearest gene. d Enriched GO terms of the acH4 peaks. e Snapshot of the raw reads results obtained in the acH4 ChIP-seq in the 30-kb region around junba (marked with a box and an arrow showing its TSS). At the top, blue lines indicate the mean reads in the control samples, while red lines indicate the mean reads in the NaBu-treated samples. At the bottom, the lines indicate the standard deviation of control and Nabu-treated samples across the region. Green box indicates the peaks detected by the algorithm. f Same as e but for the tfap2d gene. g Same as e but for the klf4b gene. h Histone H4 acetylation levels quantified in conventional ChIP as the fold change compared to the non-bound fraction in eight selected regions in control, NaBu-treated, and hdac1 +/− larvae. Blue symbols represent the acH4 ChIP, while red symbols show a control ChIP using only IgG. Bars represent standard deviation using three replicates. The legend on the right indicates the names of the regions. i Same as h but using H4K12 acetylation ChIPs. j Diagram representing cluster selection in k. Each color marks the behavioral area from which we retrieve larvae for comparison. This area depends on the distance to the average behavior of the population (r). k Same as h but comparing the acH4 differences in the different clusters represented in j