Fig. 2

DNA methylation in F1 sperm is unaffected by Dex treatment. a Pearson correlation heatmaps with hierarchical clustering for 5mC datasets from sperm from offspring in which the mother had been exposed to dexamethasone (D) or vehicle controls (V) as well as in liver (L). b Circular visualisation of average meDIP datasets plotted as heatmaps. Veh, blue bars; Dex, red bars. Change in meDIP signal between Dex and Veh are plotted in black between the heatmap data. Positions of genes are shown in the inner circle. c Box plot of 5mC signals across one of five genomic compartments (“promoter core”, TSS ± 100 bp; “promoter proximal”, TSS + 1 kb; “promoter distal”, TSS + 1 kb to + 2 kb; “genic” or “non-genic”, not associated with any of the above). d Heatmap of average promoter core 5mC levels across sample sets. e Boxplot of 5mC signals across four common classes of repetitive element. f Boxplot of standard deviation scores between sample groups across genomic compartments. g Sliding window analysis of 5mC patterns (average patterns shown in bold, upper and lower plots denote upper and lower patterns using standard deviation scores between samples. In all plots asterisks denote p value < 0.05, Wilcoxon signed-rank test