Fig. 3
From: Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging
Comparison of different dCas9/gRNA systems for labeling the endogenous human MUC4 genomic locus. a A gRNA targeting the human MUC4 gene was transfected together with dCas9 (upper panel) or without dCas9 (lower panel). b Upper panel: histograms of dCas9/gRNA foci formation efficiency in different dCas9/gRNA labeling systems (measured as % of GFP-positive cells, N > =20) in the condition with dCas9 (black bars) and without dCas9 (gray bars). Lower panel: dot plots of signal-to-noise ratio in the condition with dCas9 (black dots) and without dCas9 (gray dots). Each dot represents average value of all foci in one cell