Fig. 3

Creating small genetic change animal models using the i-GONAD method. Restoration of Tyr gene of albino Jcl:MCH(ICR) mice by single-stranded oligo donor (ssODN)-based knock-in with the i-GONAD method. a Schematic to show rescue of Tyr gene mutation. The target region containing the guide sequence and the genotyping primer binding sites are shown. b Representative E14.5 litter showing Tyr rescued G0 fetuses. The pigmented eyes of the fetuses are indicated by yellow arrows. c Representative Tyr rescued G0 mouse litters obtained from #5 female mouse in Additional file 1: Table S2. G0 mice indicated in # numbers (shown in yellow) were used for germline transmission analysis (see details in Additional file 1: Figure S2). d Direct sequencing results of PCR products amplified from the G0 fetuses in b. The positions for mutated/corrected nucleotides are indicated by red arrows. e Efficiencies of Tyr gene editing. Electroporators from three different suppliers were used (see Additional file 1: Table S2 for details)