Fig. 2
From: Kourami: graph-guided assembly for novel human leukocyte antigen allele discovery
Overview of our method. a A gene-wise MSA is obtained from the IMGT/HLA database. The reads aligning to HLA regions are extracted b from the input BAM and they are realigned c to the sequences in the MSA. d A POG is constructed from MSA and further modified via alignment projection. e Haplotype assembly of two alleles is obtained by finding two paths (drawn in red and blue; overlap in purple) through the graph. DB database, HLA human leukocyte antigen, MSA multiple sequence alignment, POG partial-order graph, WGS whole-genome sequencing