Fig. 4

SETDB1 protects IAPs from TET2-dependent loss of H4R3me2s. a, b ChIP-qPCR data for H3K4me3 and H3K9me3 (a) or H4R3me2s (b) at IAPs upon SETDB1 loss in the presence and absence of TET2 (n = 2–3; ANOVA with Sidak’s multiple comparison test, **p < 0.01, ***p < 0.001). c. Fold-change in expression upon SETDB1 knockdown for different repeat classes, depending on whether they are enriched for H4R3me2s peaks or not (n = 3, ****p < 0.0001, t-test). d ChIP-qPCR data for H4R3me2s at additional ERVs upon SETDB1 loss in the presence and absence of TET2 (n = 2; error bars represent the mean ± standard deviation). e TET2 enrichment levels at ERVs. Data are shown as mean ± standard deviation of two independent experiments