Fig. 2

TET2 drives IAP expression in the absence of SETDB1. a ChIP-qPCR data showing TET1 and TET2 enrichment at IAPs and L1 elements in primed (in the presence and absence of TETs) and naïve ESCs. Data points from two independent experiments are shown. b Expression of IAPLTR1 and IAPLTR2 in SETDB1 and TET1- or TET2-depleted cells in naïve ESCs (n = 2; one-way ANOVA, Dunnet’s multiple comparison test, *p < 0.05, **p < 0.01). Error bars represent the mean ± standard deviation. c RT-qPCR analysis of Tet2 WT, knockout (KO) and rescue cell lines at IAPs in naïve ESCs (n = 3–4; ANOVA with Tukey’s multiple comparison test, *p < 0.05, ****p < 0.0001). The results are presented as mean ± standard deviation. d Fold-change in expression between TET2 wild-type (WT) and mutant rescue lines for different repeat classes, depending on whether they are repressed by SETDB1 or not (n = 3, ****p < 0.0001, t-test)