Fig. 8
CAL27 and Detroit 562 phenotypes after down-regulation of circPVT1. a circPVT1 level was detected by RT-qPCR in siRNAcircPVT1, siRNAb-circPVT1, and siRNAct transfected cells. b–e CAL27 cell line. b Cell proliferation was determined by cell counting 24, 48, and 72 h after transfection in siRNAcircPVT1 and siRNAct transfected cells. c Propidium iodide flow cytometric assay to analyze the cell cycle in siRNAcircPVT1 and siRNAct transfected cells. d Colony formation assay in siRNAcircPVT1 and siRNAct transfected cells. Top: representative colony-forming assay. Bottom: quantification of three independent experiments by colony counting. e Migration assay in siRNAcircPVT1 and siRNAct transfected cells. f–i Detroit 562 cell line. f Colony formation assay in siRNAcircPVT1, siRNAb-circPVT1, and siRNAct transfected cells. Top: representative colony-forming assay. Bottom: quantification of three independent experiments by colony counting. g Viability of siRNAcircPVT1 and siRNAct transfected cells. h Viability after cisplatin (CDDP) treatment in siRNAcircPVT1 and siRNAct transfected cells. i The EC50 and the LD50 were determined using the Compusyn Software [58]. CDDP cisplatin, EC50 half maximum effective concentration; LC50 half lethal concentration. Data are shown as mean of three replicates ± standard deviation (Student’s test; *p < 0.05; **p < 0.01; ***p < 0.001)