Fig. 5From: The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complexThe mut-p53/YAP/TEAD complex regulates circPVT1 expression. a–h Analysis of the circPVT1 promoter. a, b ChIP analysis of p53 and TEAD1 in the CAL27 cell line. c, d ChIP analysis of YAP and Pol II in the CAL27 cell line. e–h ChIP analysis of Pol II and p-Pol II in the CAL27 cell line. i–l Analysis of the PVT1 promoter. i, j ChIP analysis of p53 and TEAD1 in the CAL27 cell line. k, l ChIP analysis of YAP and Pol II in the CAL27 cell line. m–o DRB-4sU assay. m p53, YAP, and TEAD1 proteins were detected by western blot after transfection of the indicated siRNA. GAPDH was used as internal loading control. n Nascent circPVT1 expression level was detected by RT-qPCR. o Nascent PVT1 expression level was detected by RT-qPCR. Data are shown as mean of three replicates ± standard deviation (Student’s test; *p < 0.05; **p < 0.01; ***p < 0.001)Back to article page