Fig. 1

The influence of miRNAs on AGO2, eIF4E, eIF4G, and PABP occupancies on endogenous mRNAs. a The influence of miRNAs on mRNA abundance and AGO2 occupancy, as determined by RIP with NanoString quantification. Line graphs plot the cumulative distributions of changes in total mRNA (left), AGO2-immunoprecipitated mRNA (middle), and AGO2 occupancy (right) due to transfection of miR-124 (top) or miR-155 (bottom), distinguishing the results for targets of the transfected miRNA from those of the other miRNA (red and black, respectively). Bar plots show the median fold changes in total mRNA (black), AGO2-immunoprecipitated mRNA (blue), and inferred AGO2 occupancy (purple) attributable to the indicated miRNA. To arrive at these changes, median changes for non-target mRNAs were subtracted from those of target mRNAs. Significant differences in the cumulative distributions attributable to the miRNA are indicated: *p < 0.05; **p < 0.01; ***p < 0.0001; two-tailed K–S test. b The influence of miRNAs on mRNA abundance and eIF4E, eIF4G, and PABP occupancies. Otherwise, this panel is as in a. c The influence of miRNAs on eIF4G occupancy, as determined by RIP-seq. The effects on site-containing mRNAs were calculated relative to control mRNAs without sites. Otherwise, bar graphs are as in a. d The influence of miRNAs on PABP occupancy, as determined by RIP-seq and plotted as in c. e The influence of miRNAs on eIF4E occupancy, as determined by RIP-seq and plotted as in c