Fig. 4
From: Editing inducer elements increases A-to-I editing efficiency in the mammalian transcriptome
Structural requirements for efficient editing at the Q/R site in the GluK2 transcript. a Top: the GK2Q/R construct showing the structure formed in the vicinity of the Q/R site. The edited adenosine (red dot) is located in exon 12 and pairing sequences are located within intron 12. Three stems—the Upstream stem, Q/R stem, and Downstream stem—are separated by larger internal loops. The region in grey illustrates the position of the EIE. Middle: the GK2Q/R-ΔEIE USS reporter where the upstream stem has been deleted. Bottom: the GK2Q/R-ΔEIE DSS reporter where the downstream stem has been disrupted. b Quantification of editing efficiency at the Q/R site from the different GK2Q/R constructs transfected into HeLa cells. c Quantification of editing efficiency at the Q/R site from the different GK2Q/R constructs co-transfected with ADAR2 in HEK 293 cells. The mean value of the ratio between the A and G peak heights from three individual experiments was calculated as the percentage of editing. Error bars are standard deviation