Fig. 6
From: Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage
Transcription activation is not impaired compared to the WT when the nuclease variants are charged with altered sgRNAs. Transcription activation of an EGFP inserted after the Prnp promoter, employing five targets in the promoter region. a Active nuclease variants programmed with 15-nucleotide-long truncated MS2 aptamer-containing sgRNAs. b Dead nuclease variants programmed with 21-nucleotide-long MS2 aptamer-containing sgRNAs. Spacers used are shown as combs where green represents matching and red mismatching positions; lower case g represents appended nucleotides to the 5′ end of the guide; numbering corresponds to the distance from the PAM. Bars correspond to averages of n = 3 parallel samples; error bars represent the standard deviations