Fig. 4

dCas9-SunTag-DNMT3A is capable of methylating 4.5-kb regions of HOXA5 locus with minimal effects on global methylome and transcriptome. a The methylation level of detected CpGs across the genome as determined by reduced representation bisulfite sequencing (RRBS). b The correlation between dCas9-SunTag-DNMT3A1-treated samples by Pearson’s correlation method. c The methylation status of detected CpGs at HOXA5 locus by whole genome bisulfite sequencing. The proportional methylation at each analyzed CpG is plotted from 0 to 1 (representing from 0 to 100% methylation). d The genome-wide gene expression analysis (log2 fold changes of FPKM) of dCas9-SunTag-DNMT3A1-treated samples compared to non-transduced cells (Neg) using RNA-seq. Red dot represents relative DNMT3A expression, and blue dot represents relative HOXA5 expression compared to Neg cells