Fig. 5

The absence of ADAR1-mediated editing has minimal effect on gene expression in the adult brain. RNA-sequencing (RNA-seq) analysis of 12-week-old mouse brains (n = 3 biological replicates/genotype). a MA plot for differentially expressed genes. All genes expressed in brain with CPM ≥ 2 in all replicates/genotype indicated by a dot. b Heatmap of differentially expressed genes with a log₂FC ≥ 1 in three biological replicates/genotype. Colors reflect the z-score. c Enriched pathways by GO term within differentially expressed genes (false discovery rate [FDR] ≤ 0.05, log₂FC ≥ 1, n = 29 genes). d QuSAGE analysis using the Adar1 ^E861A/E861A fetal liver gene signature as the gene set. Each gene is depicted by a single line within the barcode. Adult brain from Adar1 ^E861A/E861A Ifih1 ^-/- mice and fetal brain from Adar1 ^E861A/E861A mice (relative to respective controls; n = 3 biological replicates/genotype) were compared against the Adar1 ^E861A/E861A gene set. Curves represent the standard deviation between biological replicates for each gene and are color-coded by the magnitude of deviation. The black curve in each panel represents the average log₂FC for all genes in the set in each dataset